1) STAFF RESEARCH ASSOCIATE I, AVAILABLE IMMEDIATELY

SUMMARY OF JOB DUTIES & RESPONSIBILITIES:

Perform tissue culture, cell sorting and molecular biology experiments, including maintenance of bacterial cultures, mammalian cell lines, radiolabelling and processing of clinical specimens for diagnosis.  Perform agarose gel electrophoresis, DNA extraction, DNA cloning, DNA template preparation and quantitation for sequencing, Western blotting and general lab maintenance.  Must be willing to learn and operate complex instruments including:  centrifuges, electrophoresis apparatus, spectrophotometers and Macintosh computer.  Classwork in cell biology, microbiology or molecular biology is required; ability to learn how to execute experiments reproducibly and be a fast learner are also required.  Assistance with various procedures relating to immunizing, injecting, bleeding and harvesting samples from various laboratory animals (mice/rats) may be required.  Will sometimes require weekend work.

SKILLS, KNOWLEDGE & ABILITIES NECESSARY TO PERFORM JOB:

Background experience in PCR, gel electrophoresis, DNA cloning, excellent sterile technique, and ability to operate standard laboratory equipment are required skills; experience in processing clinical samples and handling of pathogenic bacteria are preferred skills.  Preference given to individuals with educational background in cellular biology, microbiology or molecular biology.  Proven ability to learn complex tasks and willingness to assume additional responsibilities, and must be organized, dependable, efficient and able to work/communicate well with a large number of researchers.

2) TWO WORK STUDY POSITIONS, PART-TIME, 4+ HOURS/WEEK, AVAILABLE
IMMEDIATELY

CLERK I-RESPONSIBILITIES:

Assist in projects investigating the emerging pathogen, Bartonella, that causes opportunistic infection in AIDS patients.  Work to be performed: data entry and organization, preparation of reports and compilation and maintainance of specialized resource files and slides.  Opportunities to learn about projects and interact with laboratory investigators.  Very flexible hours possible, including weekends and evenings, if desired.  Minimum: 4 hours per week.  One year commitment preferred.

LAB HELPER-RESPONSIBILITIES

Pouring media plates, assisting with basic microbiological and molecular experiments, as well a general lab maintenence.  Opportunities to learn about projects and interact with laboratory investigators, and for conducting own research project on Bartonella, if desired.  Very flexible hours possible, including weekends and evenings, if desired.  Minimum: 4 hours per week.  One year commitment preferred.  Requires prior lab experience and familiarity with sterile technique.

3) POSTDOCTORAL SCHOLAR PATHOGENESIS POSITION, AVAILABLE IMMEDIATELY

Postdoctoral position available to study the pathogenesis of Bartonella.  Projects include: a) study of the interactions between Bartonella bacilli and host cells (endothelial cells and erythrocytes); b) the regulation of gene expression in response to environmental cues such as iron availability; and 3) the role of a family of variably expressed outer membrane proteins (Vomp) in pathogenesis and immune evasion.  Experience in molecular biology and microbiology required.  Strong background in microbial genetics and/or protein purification preferred.  Send a CV, statement of career goals/interests and names of 3 references to Dr. Koehler.